Wound Healing Screening

High-throughput wound healing screening in 3D

Our screening service includes:

  • High-throughput compound screening on viability, migration, proliferation
  • Real-time closure data
  • High-content experimentation

 

Magnetic Printing

n3D offers its assay for high-throughput toxicity testing, the BiO Assay, to screen compounds for wound healing. We print cells into 3D rings that immediately respond by closing, and do so in a dose-dependent manner, which is tracked visually using our n3Dock imaging kit. Ring closure mimics wound healing and is akin to a 2D scratch assay, in which a monolayer of cells is scratched and the time it takes cells to close the wound is measured. Like the scratch assay, ring closure in this assay is related to migration, metabolism, and viability1, but in a representative 3D environment that can predict human in vivo response.

Comparison of results on the effect of ibuprofen on HEK293s using the BiO Assay (black), and a viability assay on 3D cultures (red) and 2D cultures (blue)

Comparison of results on the effect of ibuprofen on HEK293s using the BiO Assay (black), and a viability assay on 3D cultures (red) and 2D cultures (blue). 3D cultures are less sensitive to the compound because of more in vivo-like biochemical concentration gradients

Check out our publication in Scientific Reports

1. What cell types can I screen with this assay?
Our system is universally applicable to all cell types (see table for all cell types we’ve assayed). We can screen virtually any cell type, just let us know which type, and what media and environment you use to culture your cells.

2. How many compounds can I test?
We can screen compounds in multiples of 4, each with 8 concentrations and in triplicate.

3. What is the general protocol?

  1. Cells will be incubated with NanoShuttleTM-PL overnight
  2. Cells will be printed into rings the next day for 15 min – 1 h
  3. Rings will contract for 24 h and imaged at regular intervals
  4. Images batch analyzed to yield contraction data
  5. Post-assay experimentation on rings to investigate mechanisms of toxicity

4. High-content screening is possible with post-assay experiments. What can you perform?
We can do a number of post-assay experiments, including but not limited to:

  • Immunohistochemistry
  • Biochemical assays (MTT, Live/Dead, ELISA)

5. What will I receive?

  • Detailed, concise report of results presented in meaningful way
  • Online presentation and meeting to discuss results
  • Raw data, images, and movie of wound healing

Questions? Interested? Contact us for more information and to receive a quote!