Oncology Screening

Screen tumor-like spheroids in high-throughput

Our oncology screening service includes:

  • High-throughput screen for compound efficacy
  • Real-time contraction data
  • High-content experimentation
HOS

Effect of doxorubicin on HOS (human osteosarcoma) spheroids. (A) Timeseries of spheroid contraction. (B) Comparison of the BiO Assay with the MTT assay on 2D cultures. Note that with higher concentrations of doxorubicin, the spheroids are unable to contract as well. 3D spheroids show higher sensitivity to the compound than 2D monolayers with more in vivo-like biochemical concentration gradients

Using magnetic 3D bioprinting, n3D can rapidly print and screen multicellular tumor spheroids (MCTS) for oncology compound efficacy. We utilize our BiO Assay, where MCTSs contraction is related to viability and proliferation, and is captured in real time using our n3Dock imaging kit. This assay is repeatable, high-throughput, and high-content in a representative 3D environment. And unlike other spheroid systems, this assay is rapid, meaning we can screen more compounds faster.

PC3 384-well

PC3 spheroids in 384-well plates exposed to various concentrations of doxorubicin imaged by the iPod. The time-dependent (top right) and dose-dependent responses (bottom left) of PC3s to doxorubicin.

1. What cell types can I screen with this assay?
Our system is universally applicable to all cell types (see table for all the cancer cell types we’ve assayed). We can screen virtually any cancer cell type, just let us know which type, and what media and environment you use to culture your cells.

2. How many compounds can I test?
We can screen compounds in multiples of 4, each with 8 concentrations and in triplicate.

3. What is the general protocol?

  1. Cells will be incubated with NanoShuttleTM-PL overnight
  2. Cells will be printed into spheroids the next day for 15 min
  3. Spheroids will contract for at least 5 h, imaged at regular intervals for the length of the experiment
  4. Images batch analyzed to yield contraction data
  5. Post-assay experimentation on rings to investigate mechanisms of action

4. High-content screening is possible with post-assay experiments. What can you perform?
We can do a number of post-assay experiments, including but not limited to:

  • Immunohistochemistry
  • Biochemical assays (MTT, Live/Dead, ELISA)

5. What will I receive?

  • Detailed, concise report of results presented in meaningful way
  • Online presentation and meeting to discuss results
  • Raw data, images, and movie of contraction

Questions? Interested? Contact us for more information and to receive a quote!