In magnetic levitation, cells are magnetized with NanoShuttleTM-PL through overnight incubation and dispensed into a cell-repellent multiwell plate, where they are levitated off the bottom by a magnet above the plate.1 In levitating cells off the bottom of a multiwell plate, the magnetic forces work as an invisible scaffold that rapidly aggregates cells, and induces cell-cell interactions and ECM synthesis. The 3D culture is formed without any artificial substrate or specialized media or equipment and can be cultured long-term.2 Additionally, adding and removing solutions is made easy by the use of magnets to hold down 3D cultures when removing solutions, limiting culture loss. 3D cultures can also be picked up and transferred between vessels using magnetic tools such as the MagPenTM.
Magnetic levitation is an easy tool to recreate native tissue environments in vitro!
|Product||Includes||Part #||Manual||Click to Buy!|
|Single Well Bio-Assembler Kit||
|6-well Bio-Assembler Kit||
|24-well Bio-Assembler Kit||
1. Souza, G. R. et al. Three-dimensional tissue culture based on magnetic cell levitation. Nat. Nanotechnol. 5, 291–6 (2010).
2. Daquinag, A. C., Souza, G. R. & Kolonin, M. G. Adipose tissue engineering in three-dimensional levitation tissue culture system based on magnetic nanoparticles. Tissue Eng. Part C. Methods 19, 336–44 (2013).