Consumables

NanoShuttleTM-PL – Magnetize your cells!

 

  • Static incubation overnight to magnetize cells
  • Released after 8 days1
  • No effect of NanoShuttleTM-PL on metabolism, proliferation, or inflammatory stress2-4
  • No special media or equipment required
  • No interference with fluorescent microscopy, other experimental techniques

The heart of n3D’s cell culturing technology, NanoShuttleTM-PL consists of gold, iron oxide, and poly-L-lysine.1 NanoShuttleTM-PL magnetizes cells by electrostatically attaching to cell membranes during an overnight static incubation. Magnetized cells will appear peppered with dark nanoparticles after incubation. NanoShuttleTM-PL will stay attached to the cell membrane for up to 8 days, at which point it’s released into the 3D culture.1 NanoShuttleTM-PL is biocompatible, having no effect on metabolism, proliferation, and inflammatory stress2-3, and even encouraging proliferation in 3D.1,4 Additionally, it does not interfere with other experimental techniques, such as fluorescence5, or Western blotting.6

Check out our flyer on NanoShuttleTM-PL for more info!

NanoShuttleTM-PL

Product Includes Part # Manual To Pruchase
NanoShuttle 1 pack NanoShuttleTM-PL Refill
  • 600-μL Vials of NanoShuttleTM-PL (1)
005-NS CLICK HERE
NanoShuttle 3 pack NanoShuttleTM-PL Refill 3 Pack
  • 600-μL Vials of NanoShuttleTM-PL (3)
005-NS-3PK CLICK HERE
NanoShuttle 6 pack NanoShuttleTM-PL Refill 6 Pack
  • 600-μL Vials of NanoShuttleTM-PL (6)
005-NS-6PK CLICK HERE

Ask for discounts on larger volumes!

Cell-Repellent Plates

Product Includes Part # Manual Buy
Cell-Repellent 35-mm Petri Dishes
  • Cell-Repellent 35-mm Petri Dishes (10)
004-SWP CLICK HERE
Cell-Repellent 6-well Plate 008-6WP CLICK HERE
Cell-Repellent 24-well Plate 009-24WP CLICK HERE
Cell-Repellent 96-well Plate 011-96WP CLICK HERE
Cell-Repellent 384-well Plate 012-384WP CLICK HERE
96-well Deep Well Mixing Reservoir Plate 020-BIO96MXPLT CLICK HERE

*US prices only. Ask your local distributor for prices in your region.

 

References

1. Souza, G. R. et al. Three-dimensional tissue culture based on magnetic cell levitation. Nat. Nanotechnol. 5, 291–6 (2010).
2. Tseng, H. et al. Assembly of a three-dimensional multitype bronchiole coculture model using magnetic levitation. Tissue Eng. Part C. Methods 19, 665–75 (2013).
3. Tseng, H. et al. A three-dimensional co-culture model of the aortic valve using magnetic levitation. Acta Biomater. 10, 173–82 (2014).
4. Castro-Chavez, F., Vickers, K. C., Lee, J. S., Tung, C.-H. & Morrisett, J. D. Effect of lyso-phosphatidylcholine and Schnurri-3 on osteogenic transdifferentiation of vascular smooth muscle cells to calcifying vascular cells in 3D culture. Biochim. Biophys. Acta 1830, 3828–34 (2013).
5. Daquinag, A. C., Souza, G. R. & Kolonin, M. G. Adipose tissue engineering in three-dimensional levitation tissue culture system based on magnetic nanoparticles. Tissue Eng. Part C. Methods 19, 336–44 (2013).
6. Molina, J. R., Hayashi, Y., Stephens, C. & Georgescu, M.-M. Invasive glioblastoma cells acquire stemness and increased Akt activation. Neoplasia 12, 453–63 (2010).