Viability Assays & Imaging

High-throughput compound screening in 3D

 

  • Biocompatible nanoparticles1 to magnetize cells
  • 3D spheroids/rings mimicking native tissue environments
  • Rapid printing of spheroids/rings (15 min to a few hours)2
  • Shrinkage is label-free, quantitative
  • Automated imaging – set iPod and go!
  • High-throughput, high-content
  • Magnetized spheroids that are easy to handle/retrieve
  • No special equipment or media needed

Vascular “rings” of A10 rat vascular smooth muscle cells exposed to blebbistatin and norepinephrine

The current standards for compound screening are animal models; while representing human tissues of interest, these models are expensive, scarce, and carry ethical challenges. On the other end, in vitro assays poorly mimic native cellular environments and thus human in vivo response, but offer high-throughput testing with ease. Thus, there is a demand for in vitro assays that are both predictive of human in vivo response and high-throughput.

Magnetic Printing
Magnetic printing of rings

As a result, n3D developed a viability assay, the BiO Assay. Based off magnetic 3D bioprinting, cells magnetized with NanoShuttle (NS) are printed into spheroids and rings. Immediately after printing, these structures will shrink/close, as a function of cell migration, viability, and proliferation, and varies with dosage.1 Shrinkage is captured using a compact imaging kit (n3Dock) with an iPodTM programmed by a freely available app (Experiment Assistant) to image whole plates at specific intervals, forgoing the need to image well-by-well under a microscope. Shrinkage is complete within a day, and images are batch processed to rapidly yield toxicity data. Moreover, as shrinkage is label-free, the remaining rings or spheroids are available for further experimentation (IHC, Western blot, genomics, etc.)

The BiO Assay combines 3D cell culture environments with high-throughput and high-content testing to effectively predict in vivo response in vitro

n3Dock
n3Dock – iPod-based imaging

Ring v. Spheroid

The BiO Assay can be used to track the shrinkage of both rings and spheroids. While both shapes will shrink similarly and are assayed identically, the different shapes can represent different situations.

For rings, closure of the ring can represent wound-healing, wherein cells are working to close the void in the middle of the ring. Additionally, rings can represent similarly shaped tissues, like blood vessels, where dilation and contraction can be assayed (see video above).

For spheroids, shrinkage is related to spheroid assembly, with the assay macroscopically measuring how well the cells are interacting and migrating to build a competent structure (see video below).

 


Spheroids of 3T3 murine embryonic fibroblasts exposed to retinoic acid

Products

Product Includes Part # Manual Click to Buy!
96-Well BiO Assay – Complete System
  • 600-μL Vials of NanoShuttleTM-PL (3)
  • 6-Well Levitating Drive (1)
  • 96-Well Spheroid Drive (1)
  • 96-Well Holding Drive (1)
  • 96-Well Ring Drive (1)
  • 96-Well Deep Well Plate (1)
  • Cell-Repellent 6-Well Plates (2)
  • Cell-Repellent 96-Well Plates (2)
  • n3Dock (1)
  • Light Pad (1)
  • Cooling Fan (1)
  • AC-to-USB Adapter (1)
  • Extension Cord (1)
  • Analytical Software – link
020-BIOCMSYS BiO Assay
96-well BiO Assay with Imaging

n3Dock Imaging System
n3Dock

$2099
384-Well BiO Assay – Complete System
  • 600-μL Vials of NanoShuttleTM-PL (2)
  • 6-Well Levitating Drive (1)
  • 384-Well Spheroid Drive (1)
  • 384-Well Holding Drive (1)
  • 96-Well Deep Well Plate (1)
  • Cell-Repellent 6-Well Plates (2)
  • Cell-Repellent 384-Well Plates (2)
  • n3Dock (1)
  • Light Pad (1)
  • Cooling Fan (1)
  • AC-to-USB Adapter (1)
  • Extension Cord (1)
  • Analytical Software – link
020-BIOCMSYS384 BiO Assay
384-well BiO Assay

n3Dock Imaging System
n3Dock Imaging System

$2499
96-Well BiO Assay – Bioprinting Only
  • 600-μL Vials of NanoShuttleTM-PL (3)
  • 6-Well Levitating Drive (1)
  • 96-Well Spheroid Drive (1)
  • 96-Well Holding Drive (1)
  • 96-Well Ring Drive (1)
  • 96-Well Deep Well Plate (1)
  • Cell-Repellent 6-Well Plates (2)
  • Cell-Repellent 96-Well Plates (2)
020-BIOBSCSYS 96-well BiO Assay $1299
384-Well BiO Assay – Bioprinting Only
  • 600-μL Vials of NanoShuttleTM-PL (2)
  • 6-Well Magnetic Drive (1)
  • 384-Well Spheroid Drive (1)
  • 384-Well Holding Drive (1)
  • 96-Well Deep Well Plate (1)
  • Cell-Repellent 6-Well Plates (2)
  • Cell-Repellent 384-Well Plates (2)
020-BIOBSCSYS384 384-well BiO Assay Kit $1799
n3Dock Imaging System
  • n3Dock (1)
  • Light Pad (1)
  • Cooling Fan (1)
  • AC-to-USB Adapter (1)
  • Extension Cord (1)
020-BIOIMGSYS n3Dock Imaging System $799
6-Pack NanoShuttleTM-PL with FREE iPod
  • 600-μL Vials of NanoShuttleTM-PL (6)
  • FREE iPod (with purchase of either 020-BIOCMSYS or 020-BIOCMSYS384) (1)
020-NSBIOIPD $799

*US prices only. Ask your local distributor for prices in your region.
Contact us for additional parts.

References

1. Souza, G. R. et al. Three-dimensional tissue culture based on magnetic cell levitation. Nat. Nanotechnol. 5, 291–6 (2010).
2. Timm, D. M. et al. A high-throughput three-dimensional cell migration assay for toxicity screening with mobile device-based macroscopic image analysis. Sci. Rep. 3, 3000 (2013)