The current standards for compound screening are animal models; while representing human tissues of interest, these models are expensive, scarce, and carry ethical challenges. On the other end, in vitro assays poorly mimic native cellular environments and thus human in vivo response, but offer high-throughput testing with ease. Thus, there is a demand for in vitro assays that are both predictive of human in vivo response and high-throughput.
As a result, n3D developed a viability assay, the BiO Assay. Based off magnetic 3D bioprinting, cells magnetized with NanoShuttle (NS) are printed into spheroids and rings. Immediately after printing, these structures will shrink/close, as a function of cell migration, viability, and proliferation, and varies with dosage.1 Shrinkage is captured using a compact imaging kit (n3Dock) with an iPodTM programmed by a freely available app (Experiment Assistant) to image whole plates at specific intervals, forgoing the need to image well-by-well under a microscope. Shrinkage is complete within a day, and images are batch processed to rapidly yield toxicity data. Moreover, as shrinkage is label-free, the remaining rings or spheroids are available for further experimentation (IHC, Western blot, genomics, etc.)
The BiO Assay combines 3D cell culture environments with high-throughput and high-content testing to effectively predict in vivo response in vitro
The BiO Assay can be used to track the shrinkage of both rings and spheroids. While both shapes will shrink similarly and are assayed identically, the different shapes can represent different situations.
For rings, closure of the ring can represent wound-healing, wherein cells are working to close the void in the middle of the ring. Additionally, rings can represent similarly shaped tissues, like blood vessels, where dilation and contraction can be assayed (see video above).
For spheroids, shrinkage is related to spheroid assembly, with the assay macroscopically measuring how well the cells are interacting and migrating to build a competent structure (see video below).
|Product||Includes||Part #||Manual||Click to Buy!|
|96-Well BiO Assay – Complete System||
|384-Well BiO Assay – Complete System||
|96-Well BiO Assay – Bioprinting Only||
|384-Well BiO Assay – Bioprinting Only||
|n3Dock Imaging System||
|6-Pack NanoShuttleTM-PL with FREE iPod||
1. Souza, G. R. et al. Three-dimensional tissue culture based on magnetic cell levitation. Nat. Nanotechnol. 5, 291–6 (2010).
2. Timm, D. M. et al. A high-throughput three-dimensional cell migration assay for toxicity screening with mobile device-based macroscopic image analysis. Sci. Rep. 3, 3000 (2013)